p15(PAF) is an Rb/E2F-regulated S-phase protein essential for DNA synthesis and cell cycle progression.

p15(PAF) is an Rb/E2F-regulated S-phase protein essential for DNA synthesis and cell cycle progression.

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The p15(PAF)/KIAA0101 jolly rancher filled gummies protein is a proliferating cell nuclear antigen (PCNA)-associated protein overexpressed in multiple types of cancer.Attenuation of p15(PAF) expression leads to modifications in the DNA repair process, rendering cells more sensitive to ultraviolet-induced cell death.In this study, we identified that p15(PAF) expression peaks during the S phase of the cell cycle.We observed that p15(PAF) knockdown markedly inhibited cell proliferation, S-phase progression, and DNA synthesis.

Depletion of p15(PAF) resulted in p21 upregulation, especially chromatin-bound p21.We further identified that the p15(PAF) promoter contains 3 E2F-binding motifs.Loss of Rb-mediated transcriptional repression resulted in upregulated p15(PAF) expression.Binding of E2F4 and E2F6 to the p15(PAF) promoter turbo air m3f24-1-n caused transcriptional repression.

Overall, these results indicate that p15(PAF) is tightly regulated by the Rb/E2F complex.Loss of Rb/E2F-mediated repression during the G1/S transition phase leads to p15(PAF) upregulation, which facilitates DNA synthesis and S-phase progression.